IDEXX Laboratories, Inc. SNAP BVDV Antigen Test

Allow all components to come to room temperature.

Ear notch:

1. Dispense 1.5 ml of conjugate into a vial containing a single ear notch.
2. Cap vial and swirl gently to soak ear notch.
3. Incubate vial at room temperature (18 to 30 °C) for at least 10 minutes (range: 10 to 60 minutes).
4. Pour liquid contents of the vial into the sample well of the SNAP device.
5. When color first appears in the activation circle (may take 15 to 60 seconds), press firmly to activate. You will hear a distinct ‘snap.’ Note: some liquid may remain in the sample well.
6. Wait 10 minutes, then read results (range: 10 to 15 minutes).

Serum test: A heating device is needed (such as a heater block, water bath, etc.) capable of maintaining a temperature of 45 +/- 5 °C

1. Dispense 3 drops (150 µL) of serum sample into a sample tube; using a fresh pipette, dispense 6 drops (200 µL) of conjugate into the sample tube.
2. Cap the sample tube and gently invert 3 to 5 times to mix.
3. Place the sample tube in a heater block and incubate for 10 minutes at 45 °C (range: 10 to 20 minutes).
4. Pour the contents of the sample tube into the sample well of the SNAP device.
5. When color first appears in the activation circle (may take 15 to 60 seconds), press firmly to activate. You will hear a distinct ‘snap.’ Note: some liquid may remain in the sample well.
6. Wait 10 minutes, then read results (range: 10 to 15 minutes).